IFI Inmunofluorescencia indirecta IIF (Indirect immunofluorescence)

Creator:	John Cutler
Language pair:	Da Spagnolo a Inglese
Discipline:	-
Definition / notes:	Immunofluorescence A technique that uses a fluorochrome to indicate the occurrence of a specific antigen-antibody reaction. The fluorochrome labels either an antigen or an antibody. The labeled reactant is then used to detect the presence of the unlabeled reactant. The use of a labeled reactant (such as an antibody which both detects and indicates the antigen) to reveal the presence of an unlabeled one is termed direct immunofluorescence. The use of a labeled indicator antibody, which reacts with an unlabeled detector antibody that has previously reacted with an antigen, is termed indirect immunofluorescence. Substitution of a light meter for the human eye permits a quantitative measurement in immunofluorometry. See also Fluorescence; Immunoassay.
URL:	http://www.answers.com/topic/immunofluorescence